Systemic sclerosis & Raynaud's
Diversity and Epitope Spreading of Anti–RNA Polymerase III Antibodies in Systemic Sclerosis: A Potential Biomarker for Skin and Lung Involvement
Authors' conclusion
Our findings indicate a correlation between ES levels and the severity of skin sclerosis or the risk of other complications in SSc.
Study context
- Population
- Patients with systemic sclerosis (SSc) who were anti-RNAP III antibody (ARA)-positive
- Outcome
- Relationship between epitope spreading of anti-RNAP III antibodies and clinical manifestations of SSc
Methodological detailswheat germ cell-free translation system, longitudinal assessment, 17 full-length RNAP III subunit proteins and 5 truncated forms of RPC1 synthesised in vitro; autoantibodies measured in serum on antigen-binding plates
- Wheat germ cell-free translation system
- Longitudinal assessment
- 17 full-length RNAP III subunit proteins and 5 truncated forms of RPC1 synthesised in vitro; autoantibodies measured in serum on antigen-binding plates
Main finding
As reported in the abstract
- Autoantibodies against different RNAP III complex subunits found in ARA-positive patients with SSc
- Intermolecular epitope spreading (ES) indicators significantly correlated with mRSS and surfactant protein-D (ILD biomarker)
- Extent of disease on high-resolution CT or pulmonary function tests: no significant correlation
- Intramolecular ES indicator against RPC1 significantly correlated with mRSS and renal crisis
- Longitudinal ES in RNAP III complex subunits correlated with mRSS; potential disease activity biomarker
Original publication
Diversity and Epitope Spreading of Anti–RNA Polymerase III Antibodies in Systemic Sclerosis: A Potential Biomarker for Skin and Lung Involvement. Arthritis Rheumatol. Published 29 September 2024. doi:10.
Composed by AI from the published abstract and checked against it. How Briefs are made →