Lupus & antiphospholipid syndrome
Type I interferon limits interleukin-6 signalling in systemic lupus erythematosus (SLE) through shedding interleukin-6 receptors
Authors' conclusion
The combination of IL-6 and type I IFN induces shedding of CD126 to sIL-6R, shifting IL-6 signalling to trans-signalling.
Study context
- Population
- SLE patients and healthy individuals (sera and peripheral blood mononuclear cells (PBMCs)); HepG2 liver cells and transfected HEK-293 T cells
- Comparison
- SLE patients vs healthy individuals; cell stimulation with IL-6 plus IFNα
Methodological detailsin vitro cell stimulation, ELISA, flow cytometry, immunoprecipitation and western blotting
- In vitro cell stimulation
- ELISA
- Flow cytometry
- Immunoprecipitation and western blotting
Main finding
As reported in the abstract
- sIL-6R and IL-6 increased with SLE activity
- CD126 positive lymphocytes decreased in SLE
- IL-6 plus IFNα decreased CD126 on lymphocytes but increased sIL-6R in their supernatant
- IL-6 plus IFNα on HepG2 liver cells resulted in a similar sIL-6R increase
- Increased supernatant sIL-6R in HEK-293 T cells transfected with wild type CD126, not with the shedding-resistant mutant
- STAT3 phosphorylation upon IL-6 stimulation reduced
Original publication
Type I interferon limits interleukin-6 signalling in SLE through shedding interleukin-6 receptors. Rheumatology (Oxford). Published 9 July 2025. doi:10.
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