Systemic sclerosis & Raynaud's
Three Distinct Transcriptional Profiles of Monocytes Associate with Disease Activity in Scleroderma Patients
Authors' conclusion
We are the first to show that transcriptional signatures of CMs and NCMs can be used to unbiasedly stratify SSc patients and correlate with disease activity outcome measures.
Study context
- Population
- Diffuse cutaneous systemic sclerosis (dcSSc) patients from the Prospective Registry for Early Systemic Sclerosis; skin macrophages from a separate cohort
- Comparison
- SSc transcriptional groups A–C vs matched healthy controls
- Outcome
- Transcriptional profiles of classical monocytes (CMs) and NCMs and clinical associations
Methodological detailsunbiased clustering, age-, sex-, and race-matched healthy controls, RNA sequencing, external validation in published data
- Unbiased clustering
- Age-, sex-, and race-matched healthy controls
- RNA sequencing
- External validation in published data
Main finding
As reported in the abstract
- Patients clustered into 3 groups (A-C) by CM transcriptional signatures relative to controls; each group kept its signature in nonclassical monocytes (NCMs)
- Genes up-regulated in group C showed the highest expression vs other groups in SSc skin macrophages, relative to controls
- Groups B and C had worse lung function than group A; no difference in SSc skin disease at baseline
- Validation in published bulk monocyte RNA sequencing (RNA-seq) data: patients without skin disease most likely classified as group A
Parts of this Brief are withheld: the automatic checks could not confirm them against the abstract.
Original publication
Three Distinct Transcriptional Profiles of Monocytes Associate with Disease Activity in Scleroderma Patients. Arthritis Rheumatol. Published 1 February 2023. doi:10.
Composed by AI from the published abstract and checked against it. How Briefs are made →