Small extracellular vesicles derived from synovial fibroblasts contain distinct miRNA profiles and contribute to chondrocyte damage in osteoarthritis
Authors' conclusion
OA SF sEV regulate chondrocyte inflammatory and remodelling responses. OA SF sEV have unique signatures compared to parental cells which do not alter with IL-1β stimulation.
Study context
- Population
- Synovial fibroblasts (SF) and chondrocytes isolated from osteoarthritis (OA) synovial membrane and cartilage
- Participants
- 9 participants
- Comparison
- OA SF-derived small extracellular vesicles (sEV) on OA chondrocytes and cartilage explants
- Outcome
- OA chondrocyte gene expression after uptake of OA SF-derived sEV
Methodological detailsin vitro, cartilage explants, RNA-sequencing
- In vitro
- Cartilage explants
- RNA-sequencing
Main finding
As reported in the abstract
- Increased expression of catabolic gene MMP 13
- p < 0.01
- Decreased expression of anabolic genes aggrecan and COL2A1
- p < 0.01
- sEV from IL-1β stimulated OA SF: decreased aggrecan and COL2A1 expression
- p < 0.001
- sEV from IL-1β stimulated OA SF: increased SOX 9 gene expression
- p < 0.05
Original publication
Small extracellular vesicles derived from synovial fibroblasts contain distinct miRNA profiles and contribute to chondrocyte damage in osteoarthritis. Arthritis Res Ther. Published 28 September 2024. doi:10.
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