IL-40 is up-regulated in the synovial fluid and cartilage of osteoarthritis patients and contributes to the alteration of chondrocytes phenotype in vitro
Authors' conclusion
This study is the first to demonstrate the accumulation of IL-40 in OA cartilage and its up-regulation in the synovial fluid of OA patients compared to controls. In addition, extracellular IL-40 appears to play a role in promoting inflammation and cartilage destruction by driving chondrocyte behaviour towards a more aggressive phenotype.
Study context
- Population
- Osteoarthritis (OA) cartilage (paired loaded and unloaded regions, n=5), synovial fluid from OA patients and controls after knee injury, and chondrocytes in vitro
- Participants
- 31 vs 34
- Comparison
- OA vs controls after knee injury (synovial fluid IL-40); IL-40-treated vs untreated chondrocytes
- OAn = 31
- Controlsn = 34reference
- Outcome
- Synovial fluid IL-40 levels (ELISA)
Methodological detailsimmunohistochemistry, ELISA, in vitro
- Immunohistochemistry
- ELISA
- In vitro
Main finding
As reported in the abstract
- IL-40 in superficial-zone chondrocytes of OA cartilage, both loaded and unloaded explants
- Significant IL-40 positivity in transitional-zone chondrocytes only in loaded explants
- OA vs Controls: P < 0.0009
Original publication
IL-40 is up-regulated in the synovial fluid and cartilage of osteoarthritis patients and contributes to the alteration of chondrocytes phenotype in vitro. Arthritis Res Ther. Published 30 July 2024. doi:10.
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