Raman spectroscopic probe provides optical biomarkers of cartilage composition predictive of tissue function
Authors' conclusion
These data support the application of Raman-probe-derived biomarkers for molecular assessment of key ECM constituents that define cartilage properties in health and disease.
Study context
- Population
- Osteochondral blocks from bovine femoral condyles (with and without focal chondral lesion) and from 10 human knees (age 27–75)bovine and human osteochondral blocks
- Comparison
- Raman-probe-derived extracellular matrix (ECM) biomarkers vs quantitative MRI (T2/T2*) and histopathology (OARSI) for predicting cartilage function
- Outcome
- Variation of modulus accounted for
Methodological detailsex vivo bovine and human osteochondral blocks, custom arthroscopic Raman spectroscopy probe, multivariate linear decomposition
- Ex vivo bovine and human osteochondral blocks
- Custom arthroscopic Raman spectroscopy probe
- Multivariate linear decomposition
Main finding
As reported in the abstract
Raman biomarkers
71%-72%
OARSI
12-54%
T2*
15%-27%
T2
25%-30%
Further findings
Bovine tissues: variation accounted for by Raman biomarkers
- GAG content variation
- 78%
- Modulus variation
- 71%
- Depleted GAG and cartilage softening peripheral to the lesion despite no macroscopic degeneration
Human tissues: modulus variation accounted for by Raman biomarkers
- Modulus variation
- 71%
Original publication
Raman spectroscopic probe provides optical biomarkers of cartilage composition predictive of tissue function. Osteoarthritis Cartilage. Published 22 January 2025. doi:10.
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