Autoinflammatory & rare systemic diseases
Single-cell transcriptome analysis profiles cellular and molecular alterations in submandibular gland and blood in IgG4-related disease
Authors' conclusion
Our data revealed the cellular and molecular changes at the single-cell resolution of IgG4-RD
Study context
- Population
- Patients with IgG4-related disease (IgG4-RD) and matched controls; Submandibular glands (SMGs) and peripheral blood mononuclear cells (PBMCs)
- Comparison
- IgG4-RD vs matched controls
- Outcome
- Transcriptional landscapes of affected tissues and PBMCs at single-cell level
Methodological detailssingle-cell RNA sequencing, bulk RNA sequencing of PBMCs, multiplex immunofluorescence validation
- Single-cell RNA sequencing
- Bulk RNA sequencing of PBMCs
- Multiplex immunofluorescence validation
Main finding
As reported in the abstract
- Three novel subsets of tissue-resident immune cells in SMGs of IgG4-RD
- TOP2A_B and TOP2A_T cells had stemness signatures; trajectory analysis: TOP2A_B cells may differentiate into IgG4+ plasma cells, TOP2A_T cells into T follicular helper (Tfh) cells
- ICOS_PD-1_B cells with Tfh-like characteristics appeared an intermediate state from B cells to IgG4+ plasma cells
- Cellular communication patterns within immune cells and between immune and non-immune cells altered in IgG4-RD vs controls
- Infection-related pathways shared in B and T cells from SMGs and PBMCs
- CDR3 amino acid CQQSYSTPYTF expanded in PBMCs of IgG4-RD
Original publication
Single-cell transcriptome analysis profiles cellular and molecular alterations in submandibular gland and blood in IgG4-related disease. Ann Rheum Dis. Published 20 July 2023. doi:10.
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