Autoinflammatory & rare systemic diseases
Single-cell RNA sequencing of submandibular gland reveals collagen type XV-positive fibroblasts as a disease-characterizing cell population of IgG4-related disease
Authors' conclusion
Our findings suggest that collagen type XV-producing fibroblasts may represent a disease-characterizing non-immune cell population in IgG4-RD
Study context
- Population
- Patients with igG4-related disease (IgG4-RD) (submandibular glands; sera)
- Comparison
- IgG4-RD vs control subjects
- Outcome
- Demographics and characteristics of non-immune cells in IgG4-RD and potential serum biomarkers derived from non-immune cells
Methodological detailssingle-cell RNA sequencing, immunohistochemistry
- Single-cell RNA sequencing
- Immunohistochemistry
Main finding
As reported in the abstract
- Single-cell RNA sequence (scRNA-seq) revealed distinct clusters of fibroblasts, endothelial cells, ductal cells and muscle cells
- COL15A1 upregulated in IgG4-RD fibroblasts vs control subjects
- COL15A1-positive fibroblasts had a distinct transcriptome vs COL15A1-negative counterparts
- Immunohistochemistry confirmed a significant presence of collagen type XV-positive fibroblasts in IgG4-RD
- Immune-suppressive therapy in active IgG4-RD decreased serum collagen type XV
Original publication
Single-cell RNA sequencing of submandibular gland reveals collagen type XV-positive fibroblasts as a disease-characterizing cell population of IgG4-related disease. Arthritis Res Ther. Published 20 February 2024. doi:10.
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