Lupus & antiphospholipid syndrome
Sensitive detection of plasma interferon regulatory factor-5 (IRF5) by solid-phase proximity ligation assay validates IRF5 high and low subgroups in patients with systemic lupus erythematosus
Authors' conclusion
The SP-PLA enabled sensitive and specific detection of low-level IRF5 in plasma and confirmed the presence of IRF5 high and low subgroups in SLE using a quantitative method.
Study context
- Population
- Patients with systemic lupus erythematosus (SLE) from three subgroups; EDTA-plasma samplesthree SLE subgroups
- Participants
- 25 vs 25
- Comparison
- IRF5 high vs IRF5 low SLE subgroups
- IRF5 highn = 25
- IRF5 lown = 25reference
- Outcome
- Plasma IRF5 detection by solid-phase proximity ligation assay (SP-PLA)
Methodological detailsassay development and validation, solid-phase proximity ligation assay
- Assay development and validation
- Solid-phase proximity ligation assay
Performance
As reported in the abstract
IRF5 detectable in all SLE plasma samples, mean
0.63 pg/µL
SD
1.92 pg/µL
Maximum
13.54 pg/µL
Limit of detection
between 0.01 and 0.05 pg/µL
Original publication
Sensitive detection of plasma interferon regulatory factor-5 (IRF5) by solid-phase proximity ligation assay validates IRF5 high and low subgroups in patients with systemic lupus erythematosus. Scand J Rheumatol. Published 9 December 2025. doi:10.
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