Lupus & antiphospholipid syndrome
Evaluating the performance of four anti-dsDNA antibody detection methods
Authors' conclusion
CLIFT, ELISA, CLIA, and DLCM all showed impressive diagnostic efficacy in detecting anti-dsDNA antibodies. CLIFT shows a strong correlation with SLE activity and lupus nephritis.
Study context
- Population
- Serum samples from systemic lupus erythematosus (SLE) patients, non-SLE autoimmune diseases, non-AIDs and undiagnosed cases
- Participants
- 285 serum samples
- Comparison
- CLIFT vs enzyme-linked immunosorbent assay (ELISA) vs chemiluminescence immunoassay (CLIA) vs digital liquid chip method (DLCM) for anti-dsDNA antibody detection
- Outcome
- Concordance of the four anti-dsDNA methods and diagnostic efficacy in SLE
Performance
As reported in the abstract
Diagnostic performance for SLE
| Test or model | AUC (highest) | Sensitivity (highest) | Specificity (highest) |
|---|---|---|---|
| DLCM | 0.938 | 86.87% | — not stated in the abstract |
| CLIA | — not stated in the abstract | — not stated in the abstract | 94.03% |
AUC (highest)
DLCM0.938
CLIA— not stated in the abstract
Sensitivity (highest)
DLCM86.87%
CLIA— not stated in the abstract
Specificity (highest)
DLCM— not stated in the abstract
CLIA94.03%
— not stated in the abstract
- Overall concordance of CLIFT with other methods (ROC-derived cut-offs), kappa > 0.6, P < 0.001
- 80.14% to 82.58%
- Concordance between quantitative methods, kappa > 0.8, P < 0.001
- 89.55% to 91.29%
Further findings
Correlation with SLE disease activity index (SLEDAI) and LN
- CLIFT and DLCM correlated well with SLEDAI
- CLIFT and CLIA significantly correlated with LN
Original publication
Evaluating the performance of four anti-dsDNA antibody detection methods. Lupus. Published 23 August 2025. doi:10.
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