Lupus & antiphospholipid syndrome
A comparison of the chemiluminescence immunoassay and Crithidia luciliae immunofluorescence test in detecting anti-dsDNA antibodies and assessing the activity of systemic lupus erythematosus
Authors' conclusion
Good correlation and agreement were found between YHLO CLIA and CLIFT. In addition, there was a significant correlation between YHLO CLIA and the SLE Disease Activity Index, which was superior to that of CLIFT.
Study context
- Population
- 208 patients with systemic lupus erythematosus (SLE), 110 other autoimmune patients, 70 infectious disorders patients and 105 healthy people208 SLE patients
- Comparison
- YHLO chemiluminescence immunoassay (CLIA) vs Crithidia luciliae immunofluorescence test (CLIFT) for anti-dsDNA detection and correlation with SLE activity
- Outcome
- Anti-dsDNA detection
Performance
As reported in the abstract
| Test or model | Sensitivity | Specificity |
|---|---|---|
| YHLO CLIA | 58.2% | 95.1% |
| CLIFT | 55.3% | 99.3% |
Sensitivity
YHLO CLIA58.2%
CLIFT55.3%
Specificity
YHLO CLIA95.1%
CLIFT99.3%
- Overall agreement YHLO CLIA vs CLIFT, 160/208
- 76.9%
- Kappa (moderate correlation), p < 0.001
- 0.530
- Spearman's correlation, YHLO CLIA quantitative results vs CLIFT titers, p < .01
- 0.59
Further findings
| Outcome | YHLO CLIA | CLIFT |
|---|---|---|
| Spearman's correlation with SLEDAI-2K | ||
| R | 0.66 | 0.60 |
| p | p < .01 | p < .01 |
- YHLO CLIA at cut-off 24 IU/mL
- 66.8% · sensitivity · specificity 93.6%
Original publication
A comparison of the chemiluminescence immunoassay and Crithidia luciliae immunofluorescence test in detecting anti-dsDNA antibodies and assessing the activity of systemic lupus erythematosus. Lupus. Published 24 May 2023. doi:10.
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