Lupus & antiphospholipid syndrome
Clinical implications of discordance between anti-dsDNA antibodies by multiplex flow immunoassay andCrithidia luciliaeassay in a multiethnic racial cohort of patients with SLE
Authors' conclusion
Our data demonstrate that discordance of positivity between both assays for anti-dsDNA is relatively common, occurring in a fifth of patients overall and a third of visits. EIA positivity is associated with LN less often than CLIFT positivity.
Study context
- Population
- Patients with SLE fulfilling ACR, SLICC and/or ACR/EULAR classification criteria, in a well-phenotyped multiethnic/racial cohort586 paired results
- Participants
- 207 patients
- Exposure
- Anti-dsDNA by multiplex enzyme immunoassay (EIA) vs Crithidia luciliae immunofluorescence test (CLIFT)
- Outcome
- Concordance or discordance of paired anti-dsDNA results by EIA and CLIFT
Methodological detailsmultiethnic/racial cohort, paired simultaneous testing
- Multiethnic/racial cohort
- Paired simultaneous testing
Main finding
As reported in the abstract
Concordance of paired anti-dsDNA results
Concordant pairs
377
Discordant pairs
209
Patients always with discordant paired results (of 207)
41
Patients always with titre discordance
39
Observational data: associations, not effects.
Further findings
Positivity in patients with LN (n=100)
Sensitivity for LN
Specificity for LN
Flare prediction (42 patients with flare assessment within 90 days of paired result)
- Mild flares with concordant positive results
- 6 of 7
- Severe flares with concordant positive results
- all four
Original publication
Clinical implications of discordance between anti-dsDNA antibodies by multiplex flow immunoassay andCrithidia luciliaeassay in a multiethnic racial cohort of patients with SLE. Lupus Sci Med. Published 14 November 2023. doi:10.
Composed by AI from the published abstract and checked against it. How Briefs are made →