Lupus & antiphospholipid syndrome
Overexpression of IRF8 and Pu.1 in B cells of systemic lupus erythematosus patients
Authors' conclusion
IRF4, IRF8, and PU.1 expression in B cells is altered in SLE. PU.1 shows a positive correlation with SLEDAI and anti-dsDNA titers.
Study context
- Population
- B cells of SLE patients
- Outcome
- mRNA expression of IRF4, IRF8, SP1 and PU.1 in B cells at baseline and after B cell activation
Methodological detailsin vitro B cell activation with anti-IgM, reverse transcription polymerase chain reaction (RT-PCR)
- In vitro B cell activation with anti-IgM
- RT-PCR
Main finding
As reported in the abstract
Transcription factor mRNA expression in B cells
- IRF4 expression increased significantly in SLE upon B cell activation; no changes between groups at baseline
- IRF8 expression significantly raised in active SLE and increased upon activation
- SP1 expression stable across all groups and conditions
- PU.1 expression higher in active SLE at baseline and increased further upon B cell activation
Further findings
Correlations between transcription factors and with clinical parameters
- Positive correlations between IRF4 and IRF8, and between PU.1 and SP1
- PU.1 expression correlated with SLE disease activity indices (positive correlation with SLEDAI and anti-dsDNA titers)
Original publication
Overexpression of IRF8 and Pu.1 in B cells of systemic lupus erythematosus patients. Lupus. Published 5 August 2025. doi:10.
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