Muscle Tissue Transcriptome of Idiopathic Inflammatory Myopathy Reflects the Muscle Damage Process by Monocytes and Presence of Skin Lesions
Authors' conclusion
Our study highlights a significant involvement of monocytes in muscle damage and delineates pathologic differences among IIM subtypes. DM was characterized by complement and interferon-α and γ signaling, whereas ARS-DM was associated with OXPHOS.
Study context
- Population
- Patients with idiopathic inflammatory myopathy (IIM) (dermatomyositis (DM) or PM) with muscle biopsy samples
- Participants
- 16 / 9 / 7
- Comparison
- DM vs PM; ARS-DM within DM
- DMn = 16
- PMn = 9reference
- ARS-DMn = 7
- Outcome
- Transcriptomic and immunophenotypic features of IIM muscle
Methodological detailsbulk RNA-sequencing, weighted gene coexpression network analysis (WGCNA), differentially expressed gene (DEG) analysis, gene set variation analysis, deconvolution
- Bulk RNA-sequencing
- WGCNA
- DEG analysis
- Gene set variation analysis
- Deconvolution
Main finding
As reported in the abstract
- Significant positive correlations between serum creatine kinase (CK) and gene modules of cellular respiration, phagocytosis and oxidative phosphorylation (OXPHOS)
- Significant positive correlations between CK and proportions of CD16-positive and negative monocytes and myeloid dendritic cells
- DM: enrichment of complement and interferon-α and γ pathway genes vs PM
- ARS-DM: higher proportion of Th1 cells and DEGs related to OXPHOS
- Serum Krebs von den Lungen-6 correlated with gene modules of extracellular matrix and TGF-β signaling pathway
Original publication
Muscle Tissue Transcriptome of Idiopathic Inflammatory Myopathy Reflects the Muscle Damage Process by Monocytes and Presence of Skin Lesions. Arthritis Rheumatol. Published 3 September 2024. doi:10.
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