Multiparametric Profiling of Circulating Immune Cells Identifies an Expansion of CD25high Switched Memory B Cells in Osteoarthritis
Authors' conclusion
A CD25hi switched memory B cell population was identified to be a potential cellular biomarker for OA that can be detected in the early stages of OA in the readily accessible circulating blood cells.
Study context
- Population
- Healthy donors, patients with osteoarthritis (OA) and patients with degenerative meniscal tears
- Participants
- 21 / 17 / 10
- Comparison
- Peripheral blood mononuclear cells (PBMCs) of patients with OA and patients with degenerative meniscal tears (DMTs) vs healthy donors
- Healthy donorsn = 21reference
- OAn = 17
- DMTn = 10
- Outcome
- OA-associated features in circulating immune cells (PBMC immunophenotyping at single-cell resolution)
Methodological detailsmass cytometry by time-of-flight (29-marker panel), single-cell RNA sequencing
- Mass cytometry by time-of-flight (29-marker panel)
- Single-cell RNA sequencing
Main finding
As reported in the abstract
Circulating immune cell perturbations, OA vs healthy donors
- Subsets of naive B cells depleted in OA
- Switched memory B cells significantly expanded in OA, incl. a CD25hiCXCR5hiCD27+IgD- subpopulation
- Dysfunction of IL-2/Stat5 and TNF signaling in CD25hi switched memory B cells in OA (single-cell RNA sequencing)
Further findings
- Similar expansion of CD25hi switched memory B cells in DMT
Original publication
Multiparametric Profiling of Circulating Immune Cells Identifies an Expansion of CD25high Switched Memory B Cells in Osteoarthritis. Arthritis Rheumatol. Published 26 May 2025. doi:10.
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