Sjögren's disease & overlap diseases
Molecular B-cell clonality assay in minor salivary glands as a useful tool for the lymphoma risk assessment in Sjögren's syndrome
Authors' conclusion
The detection of MSG monoclonal B-cell expansion by this easy-to-perform molecular assay is useful, both at the time of diagnosis and during the course of SS. Monoclonal B-cell expansion is associated with a subset of SS patients presenting either ongoing lymphoma or other established lymphoma predictive factors.
Study context
- Population
- Patients with suspected Sjögren's syndrome (SS) or suspected lymphoma during SS; fresh-frozen minor salivary glands (MSG)
- Participants
- 207 patients
- Exposure
- SS vs no SS
- SSn = 123
- Without SSn = 84reference
- Outcome
- Prevalence of clonal immunoglobulin gene rearrangements (monoclonal B-cell infiltration) in MSG
Methodological detailsstandardised multiplex PCR assay with heteroduplex analysis by microcapillary electrophoresis, EuroClonality consortium guidelines
- Standardised multiplex PCR assay with heteroduplex analysis by microcapillary electrophoresis
- EuroClonality consortium guidelines
Main finding
As reported in the abstract
MSG monoclonal B-cell infiltration
P < 0.001
- MSG monoclonal B-cell infiltration, all patients
- 31 (15%)
Observational data: associations, not effects.
Further findings
Correlates of MSG monoclonality in SS
- Correlated significantly with ongoing salivary gland non-Hodgkin's lymphoma (NHL), salivary gland swelling, CD4+ T-cell lymphopenia, rheumatoid factor (RF) activity, low complement and type 2 mixed cryoglobulinemia
Probability of MSG B-cell monoclonality by accumulation of biological risk factors
- Only positive RF
- no probability
- RF-positive with 1 other risk factor
- 25.0%
- RF-positive with 2 other risk factors
- 85.7%
Original publication
Molecular B-cell clonality assay in minor salivary glands as a useful tool for the lymphoma risk assessment in Sjögren's syndrome. Joint Bone Spine. Published 29 December 2023. doi:10.
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