Age‐Associated Changes in Knee Osteoarthritis, Pain‐Related Behaviors, and Dorsal Root Ganglia Immunophenotyping of Male and Female Mice
Authors' conclusion
We found that aging in male and female mice is accompanied by mild knee OA, mechanical sensitization, and changes to immune cell populations in the DRG, suggesting novel avenues for development of OA therapies.
Study context
- Population
- Male and female C57BL/6 mice; human dorsal root ganglion (DRGs) from individuals >80 years of age6 human DRG donors
- Comparison
- Mice 20 months vs 6 months of age, male and female
Methodological detailspreclinical (mouse), both sexes, flow cytometry, human DRG gene expression
- Preclinical (mouse)
- Both sexes
- Flow cytometry
- Human DRG gene expression
Main finding
As reported in the abstract
- Male mice at 20 months had worse cartilage degeneration than 6-month-old mice
- Older female knees showed increased cartilage degeneration, to a lesser degree than males
- Older mice of both sexes had worse mechanical allodynia, knee hyperalgesia and grip strength vs younger mice
- Both sexes: DRGs from older mice showed decreased CD45+ cells and significant increase in F4/80+ macrophages and CD11c+ dendritic cells
- Older male DRGs: increased Ccl2 and Ccl5 expression; older female DRGs: increased Cxcr4 and Ccl3 expression vs 6-month-old DRGs
- Human DRGs (6 individuals >80 years): CCL2 elevated in men vs women; CCL3 higher in women
Original publication
Age‐Associated Changes in Knee Osteoarthritis, Pain‐Related Behaviors, and Dorsal Root Ganglia Immunophenotyping of Male and Female Mice. Arthritis Rheumatol. Published 14 July 2023. doi:10.
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