TRIM63 and Atrogin-1 are key drivers of systemic and muscle inflammation in patients with idiopathic inflammatory myopathies
Authors' conclusion
TRIM63 and Atrogin-1 are expressed in PBMC and muscle from patients with IIM and correlate with serum cytokines, and chemokines.
Study context
- Population
- Adult patients with idiopathic inflammatory myopathies (IIM) and age and sex-matched healthy donors
- Participants
- 37 vs 10
- Comparison
- Adult IIM patients vs age and sex-matched healthy donors
- IIMn = 37
- Healthy donorsn = 10reference
- Outcome
- Expression of TRIM63 and Atrogin-1 (proportion of peripheral blood mononuclear cells (PBMC) subsets expressing them, serum and muscle levels) and relationship with inflammatory and atrophy parameters
Methodological detailsage and sex-matched healthy donors, multiparametric flow-cytometry, ELISA, luminometry, confocal microscopy
- Age and sex-matched healthy donors
- Multiparametric flow-cytometry
- ELISA
- Luminometry
- Confocal microscopy
Main finding
As reported in the abstract
- IIM
- TRIM63+ CD4+ T cells 24.56 (7.71-53.23) vs. 2.55 (0.42-4.51); P < 0.0001
- IIM
- TRIM63+ CD8+ T cells 15.1 (3.22-37.40) vs. 1.06 (0.83-2.45); P = 0.0002
- IIM
- TRIM63+ monocytes 14.09 (3.25-29.80) vs. 1.97 (0.59-7.64); P = 0.011
- IIM
- Atrogin-1+ CD4+ T cells 27.30 (6.61-64.19) vs. 2.55 (0.42-4.51); P < 0.0001
- IIM
- Atrogin-1+ CD8+ T cells 14.88 (5.99-34.30) vs. 2.33 (0.60-8.01); P = 0.001
- IIM
- Atrogin1+ monocytes 17.38 (8.93-47.37) vs. 1.41 (0.79-3.77); P < 0.0001
Further findings
Muscle expression of TRIM63 and Atrogin-1
- Muscle from IIM patients had a higher expression of TRIM63 and Atrogin-1
Correlation of TRIM63+ CD8+ T cells with serum cytokines
- TRIM63+ CD8+ T cells mainly correlated with serum IL-2, IL-4, IL-8, IL-10, G-CSF, and TNF-a
- Spearman Rho
Original publication
TRIM63 and Atrogin-1 are key drivers of systemic and muscle inflammation in patients with idiopathic inflammatory myopathies. Clin Exp Rheumatol. Published 9 September 2024. doi:10.
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