Systemic sclerosis & Raynaud's
Single-cell analysis reveals key differences between early-stage and late-stage systemic sclerosis skin across autoantibody subgroups
Authors' conclusion
We have identified cellular differences between the two main autoantibody subsets in dcSSc (ARA+ and ATA+).
Study context
- Population
- Patients with diffuse cutaneous systemic sclerosis (dcSSc) and healthy controls (HCs); 4 mm forearm skin biopsies15 forearm skin biopsies analysed
- Participants
- 12 vs 3
- Comparison
- dcSSc patients by stage and autoantibody vs HCs
- dcSScn = 12
- HCsn = 3reference
Methodological detailssingle-cell RNA sequencing, droplet-based sequencing (10× genomics)
- Single-cell RNA sequencing
- Droplet-based sequencing (10× genomics)
Main finding
As reported in the abstract
- Clear separation of SSc samples, by disease, stage and antibody, for all cells and fibroblast subclusters
- Differing cell cluster gene expression profiles between anti-topoisomerase antibody (ATA)+ and ARA+ patients
- Cell-to-cell interaction suggests differing interactions between early and late stages of disease and autoantibody
- TGFβ response mainly in fibroblasts and smooth muscle cells in early ATA+ dcSSc skin; in early ARA+ dcSSc skin the responding cells were endothelial
Original publication
Single-cell analysis reveals key differences between early-stage and late-stage systemic sclerosis skin across autoantibody subgroups. Ann Rheum Dis. Published 14 August 2023. doi:10.
Composed by AI from the published abstract and checked against it. How Briefs are made →