Serum proteomic biomarkers diagnostic of knee osteoarthritis
Authors' conclusion
No more than 8 serum protein biomarkers were required to discriminate knee OA from non-OA.
Study context
- Population
- Baseline sera: National Institutes of Health (NIH) cohort Kellgren-Lawrence (KL) grade 1–3 knee osteoarthritis (OA) participants and Johnston County OA Project multi-joint controls free of radiographic OA of hands, hips, knees (bilateral KL=0) and spine596 OA · spine 127
- Comparison
- Knee OA vs multi-joint non-OA controls
- Outcome
- Diagnosis of knee OA vs non-OA: ROC-AUC and PR-AUC
Methodological detailsmultiple reaction monitoring mass spectrometry, two cohorts, 70% training / 30% testing split, random forest selection, multivariable logistic regression
- Multiple reaction monitoring mass spectrometry
- Two cohorts
- 70% training / 30% testing split
- Random forest selection
- Multivariable logistic regression
Performance
As reported in the abstract
Discrimination of knee OA from non-OA
| Test or model | ROC-AUC (testing set) | PR-AUC (testing set) | ROC-AUC | PR-AUC |
|---|---|---|---|---|
| Final model (8 serum peptides) | 0.833 (95% CI 0.751, 0.905) | 0.929 (95% CI 0.876, 0.973) | — not stated in the abstract | — not stated in the abstract |
| ACTG | — not stated in the abstract | — not stated in the abstract | 0.705 | 0.897 |
| Body mass index (BMI) | — not stated in the abstract | — not stated in the abstract | 0.57 | 0.812 |
ROC-AUC (testing set)
Final model (8 serum peptides)0.833 (95% CI 0.751, 0.905)
ACTG— not stated in the abstract
Body mass index (BMI)— not stated in the abstract
PR-AUC (testing set)
Final model (8 serum peptides)0.929 (95% CI 0.876, 0.973)
ACTG— not stated in the abstract
Body mass index (BMI)— not stated in the abstract
ROC-AUC
Final model (8 serum peptides)— not stated in the abstract
ACTG0.705
Body mass index (BMI)0.57
PR-AUC
Final model (8 serum peptides)— not stated in the abstract
ACTG0.897
Body mass index (BMI)0.812
— not stated in the abstract
Further findings
Final model in non-symptomatic and symptomatic cases
- Equally diagnostic of non-symptomatic and symptomatic cases (AUCs)
- 0.830-0.835
Random forest (RF) selection
- Peptides selected as diagnostic of OA (with BMI)
- 23 peptides (19 proteins)
- STRING database predicted multiple high confidence interactions of the 19 diagnostic OA proteins
Original publication
Serum proteomic biomarkers diagnostic of knee osteoarthritis. Osteoarthritis Cartilage. Published 19 September 2023. doi:10.
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