Spondyloarthritis & psoriatic arthritis
Piezo1-mediated mechanotransduction promotes entheseal pathological new bone formation in ankylosing spondylitis
Authors' conclusion
Piezo1-mediated mechanotransduction promotes entheseal pathological new bone formation through CaMKII signalling in AS.
Study context
- Population
- Spinal ligament tissues from patients with ankylosing spondylitis (AS) and non-AS controls; collagen antibody-induced arthritis animal models; entheseal osteo-chondral lineage cells
- Participants
- 14 vs 14
- Comparison
- AS vs non-AS controls; Piezo1 inhibition or activation in animal models
- ASn = 14
- Non-AS controlsn = 14reference
Methodological detailsbulk RNA sequencing, collagen antibody-induced arthritis models, pharmacological inhibition and genetic ablation of Piezo1, in vitro cell culture
- Bulk RNA sequencing
- Collagen antibody-induced arthritis models
- Pharmacological inhibition and genetic ablation of Piezo1
- In vitro cell culture
Main finding
As reported in the abstract
- Piezo1 aberrantly upregulated in ligaments and entheseal tissues from patients with AS and animal models
- Pharmaceutical and genetic inhibition of Piezo1 attenuated, while activation of Piezo1 promoted, pathological new bone formation
- Activation of CaMKII signalling essential for Piezo1-mediated mechanotransduction
- Piezo1 upregulated by AS-associated inflammatory cytokines
Original publication
Piezo1-mediated mechanotransduction promotes entheseal pathological new bone formation in ankylosing spondylitis. Ann Rheum Dis. Published 21 December 2022. doi:10.
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