Non-inflammatory macrophages phagocytose and hydrolyse monosodium urate crystals in different stages of gout
Authors' conclusion
The efficient phagocytosis and hydrolysis of MSU crystals by M0 macrophages suggest their role in maintaining the non-inflammatory stage of gout.
Study context
- Population
- Patients in different stages of gout (asymptomatic hyperuricaemia, intercritical, tophaceous) and normal controls; M0 macrophages derived from monocytes with macrophage colony-stimulating factor (M-CSF) for 10 days
- Comparison
- Non-inflammatory (M0) macrophages from patients with asymptomatic hyperuricaemia, intercritical gout, tophaceous gout vs normal controls
- Outcome
- Phagocytosis, hydrolysis and subsequent cytokine secretion of MSU crystals by M0 macrophages (flow cytometry, side-scatter area increase)
Methodological detailsin vitro, flow cytometry
- In vitro
- Flow cytometry
Main finding
As reported in the abstract
- M0 macrophages phagocytosed and hydrolysed monosodium urate (MSU) crystals in various stages of gout, including the control group
- Asymptomatic hyperuricaemia showed the most pronounced phagocytic and hydrolytic capabilities, surpassing normal controls
- MSU alone did not induce pro-inflammatory cytokine secretion
- With lipopolysaccharide (LPS) and/or MSU stimulation, phagocytic and hydrolytic abilities correlated with inflammatory cytokine elevation
Original publication
Non-inflammatory macrophages phagocytose and hydrolyse monosodium urate crystals in different stages of gout. Scand J Rheumatol. Published 8 May 2025. doi:10.
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