Macrophage subpopulations in pediatric patients with lupus nephritis and other inflammatory diseases affecting the kidney
Authors' conclusion
Although M1-like macrophages play a greater role in pediatric LN patients than in adult LN patients, M2-like macrophages appear to be key players and are more abundant in other pediatric inflammatory kidney diseases compared to LN.
Study context
- Population
- Pediatric patients with lupus nephritis (LN) (ISN/RPS classes II–V) and pediatric controls with other inflammatory kidney diseases
- Comparison
- Pediatric LN vs pediatric controls with other inflammatory kidney diseases (and vs adult LN)
- Pediatric LNn = 20
- Outcome
- Renal infiltration with M1-like, M2a-like and M2c-like macrophages, CD3+ T-cells, CD20+ B-cells and MPO+ neutrophils
- Follow-up
- Time of renal biopsy
Methodological detailsimmunofluorescence microscopy of renal biopsies, comparison with data from a former study of adult LN patients
- Immunofluorescence microscopy of renal biopsies
- Comparison with data from a former study of adult LN patients
Main finding
As reported in the abstract
- In pediatric LN, macrophage subtype frequency depended on ISN/RPS class: most pronounced M1-like infiltration in class IV; M2c-like most abundant in class III and IV
- On average only half as many macrophages in pediatric LN biopsies vs adult LN
- Distribution of M2a-like (CD68+CD206+) differed, but M1-like (CD68+CD163-) and M2c-like (CD68+CD163+) comparable, pediatric vs adult LN
- Fewer macrophages and other inflammatory cells in LN vs other pediatric inflammatory kidney diseases
- Disease-specific inflammatory signatures could not be confirmed due to small number of pediatric cases
Further findings
Correlation of immune cell infiltration with renal function · time of renal biopsy
- Elevated serum urea and decreased eGFR correlated particularly strongly with CD68+/CD163- M1-like macrophages and CD20+ B cells in pediatric inflammatory kidney disease
Original publication
Macrophage subpopulations in pediatric patients with lupus nephritis and other inflammatory diseases affecting the kidney. Arthritis Res Ther. Published 8 February 2024. doi:10.
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