Lupus & antiphospholipid syndrome
Low-density granulocytes and neutrophil extracellular trap formation are increased in incomplete systemic lupus erythematosus
Authors' conclusion
These results show that neutrophil dysfunction, including higher proportions of LDGs, and increased NET formation, already occur in iSLE, similar to SLE, despite differences in disease manifestations.
Study context
- Population
- Patients with incomplete SLE (iSLE) and SLE, and healthy controlsLow-density granulocytes (LDGs): 18 iSLE, 11 SLE, 14 healthy controls (HCs)
- Exposure
- iSLE vs SLE vs healthy controls
- Outcome
- Proportion of LDGs (CD14lowCD15+), circulating plasma NETs and serum-induced NET formation
Methodological detailsflow cytometry, ELISA, fluorescence microscopy
- Flow cytometry
- ELISA
- Fluorescence microscopy
Main finding
As reported in the abstract
LDGs, circulating plasma NETs and serum-induced NET formation
- Proportions of LDGs and circulating plasma neutrophil extracellular traps (NETs) similarly elevated in iSLE and SLE vs HCs
- Serum from iSLE and SLE similarly induced NET formation in healthy neutrophils
Observational data: associations, not effects.
Further findings
LDGs by HCQ treatment
- Patients under HCQ had lower proportions of LDGs than those without
Correlations in iSLE
- Myeloperoxidase-DNA complexes correlated with age-associated B-cells, memory B-cells and negatively with naïve B-cells
- No associations between NET measures or serum-induced NET formation and interferon score or clinical parameters
Original publication
Low-density granulocytes and neutrophil extracellular trap formation are increased in incomplete systemic lupus erythematosus. Rheumatology (Oxford). Published 22 May 2024. doi:10.
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