Systemic sclerosis & Raynaud's
Long non-coding RNA H19X as a regulator of mononuclear cell adhesion to the endothelium in systemic sclerosis
Authors' conclusion
lncRNA H19X may contribute to EC activation in SSc vasculopathy, acting as a regulator of expression of adhesion molecules in ECs.
Study context
- Population
- SSc skin biopsies (single-cell RNA sequencing (scRNA-seq) data) and human dermal microvascular endothelial cell (ECs) (human dermal microvascular ECs (HDMECs))
- Comparison
- H19X knockdown vs control in HDMECs; H19Xpos vs H19Xneg cells; SSc vs healthy ECs
Methodological detailsscRNA-seq analysis of SSc skin biopsies, in vitro silencing in HDMECs
- scRNA-seq analysis of SSc skin biopsies
- In vitro silencing in HDMECs
Main finding
As reported in the abstract
- Significant upregulation of H19X in SSc compared with healthy ECs (scRNA-seq)
- H19X consistently induced by IFN type I and II in HDMECs
- H19X knockdown led to a significant decrease in the mRNA of several adhesion molecules; VCAM1 significantly reduced at protein and mRNA levels
- Higher expression of VCAM1 in H19Xpos ECs; ECs strongly associated with the 'cell adhesion molecule' pathway
- VCAM1 downstream pathway less activated following H19X knockdown
- Contractility of HDMECs, PBMC adhesion to HDMECs and VCAM1 shedding reduced following H19X knockdown
Original publication
Long non-coding RNA H19X as a regulator of mononuclear cell adhesion to the endothelium in systemic sclerosis. Rheumatology (Oxford). Published 1 February 2024. doi:10.
Composed by AI from the published abstract and checked against it. How Briefs are made →