Systemic sclerosis & Raynaud's
Human dermal fibroblast-derived exosomes induce macrophage activation in systemic sclerosis
Authors' conclusion
In this work, we demonstrate for the first time that human SSc dermal fibroblasts mediate macrophage activation through exosomes.
Study context
- Population
- Skin-biopsy fibroblasts from SSc patients and healthy age and gender-matched controls; human donor-derived macrophages
- Participants
- 7 vs 6
- Comparison
- Macrophages cultured with exosomes from SSc dermal fibroblasts vs exosomes from healthy control fibroblasts
- SScn = 7
- Healthy controln = 6reference
- Outcome
- Macrophage activation (surface markers and cytokine secretion) by SSc vs healthy control dermal fibroblast-derived exosomes
- Follow-up
- 48 h
Methodological detailsin vitro, age and gender-matched controls, Transwell co-culture
- In vitro
- Age and gender-matched controls
- Transwell co-culture
Main finding
As reported in the abstract
- Macrophages activated with SSc fibroblast-derived exosomes upregulated surface CD163, CD206, MHC Class II and CD16 vs healthy control (n = 7)
- P < 0.05
- Macrophages activated with SSc fibroblast-derived exosomes secreted increased IL-6, IL-10, IL-12p40 and TNF vs healthy control (n = 7)
- P < 0.05
Original publication
Human dermal fibroblast-derived exosomes induce macrophage activation in systemic sclerosis. Rheumatology (Oxford). Published 10 August 2022. doi:10.
Composed by AI from the published abstract and checked against it. How Briefs are made →