Systemic sclerosis & Raynaud's
Autoantibodies to nuclear valosin-containing protein-like protein: systemic sclerosis-specific antibodies revealed by in vitro human proteome
Authors' conclusion
Anti-NVL Ab is an SSc-specific autoantibody associated with a unique combination of clinical features, including limited skin sclerosis and lack of lung involvement.
Study context
- Population
- Systemic sclerosis (SSc) patients with nucleolar patterned ANA of unknown specific antibodies (Abs); controls: SSc with known SSc-specific autoantibodies, other connective tissue diseases, healthy subjects
- Comparison
- Anti-NVL Ab-positive SSc vs SSc with NUC-ANAs other than anti-NVL Abs
- Outcome
- Identification of undescribed SSc-specific autoantibodies targeting nucleolar antigens and their clinical significance
Methodological detailsproteome-wide autoantibody screening (PWAS), wet protein arrays from in vitro human proteome, retrospective review of electronic medical records
- Proteome-wide autoantibody screening (PWAS)
- Wet protein arrays from in vitro human proteome
- Retrospective review of electronic medical records
Main finding
As reported in the abstract
- PWAS identified three candidate autoantibodies, including anti-NVL Ab
- Only anti-NVL Abs exclusively detected in SSc vs disease controls
- Anti-NVL Ab detection reproduced by immunoblotting and immunoprecipitation
- Indirect immunofluorescence: homogeneous nucleolar patterns
Further findings
Clinical features: diffuse skin sclerosis and ILD
- Significantly low prevalence of diffuse skin sclerosis and ILD in anti-NVL Ab-positive cases vs SSc with other NUC-ANAs
Original publication
Autoantibodies to nuclear valosin-containing protein-like protein: systemic sclerosis-specific antibodies revealed by in vitro human proteome. Rheumatology (Oxford). Published 30 January 2024. doi:10.
Composed by AI from the published abstract and checked against it. How Briefs are made →