Insights into the detection of AMPA cross-reactivity: comparing cyclic peptide- to protein-based assays
Authors' conclusion
Antigen backbones influence the detection of AMPA cross-reactivity.
Study context
- Population
- Rheumatoid arthritis (RA) patients from the Early Arthritis Clinic (EAC)
- Participants
- 608 patients
- Exposure
- Cyclic peptide-based vs modified fetal calf serum (FCS)-based enzyme-linked immunosorbent assays (ELISAs) for AMPA reactivity
- Outcome
- Samples reacting to both citrullinated and carbamylated PTM residues
Methodological detailsELISA comparison of modified FCS- vs cyclic peptide (CXP2)-based assays, AMPA isolation and cross-reactivity assessment
- ELISA comparison of modified FCS- vs cyclic peptide (CXP2)-based assays
- AMPA isolation and cross-reactivity assessment
Main finding
As reported in the abstract
Observational data: associations, not effects.
Further findings
AMPA isolation by antigen backbone
- Modified FCS captures anti-modified protein antibodies (AMPAs) with a more restricted, less cross-reactive epitope recognition profile than modified peptides
Original publication
Insights into the detection of AMPA cross-reactivity: comparing cyclic peptide- to protein-based assays. Arthritis Res Ther. Published 7 July 2025. doi:10.
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