Systemic sclerosis & Raynaud's
Immunoglobulins G from Patients with Systemic Sclerosis Modify the Molecular Signatures of Endothelial Cells
Authors' conclusion
Purified IgG from patients with SSc can modify EC proteome and transcriptome. The observed changes closely associate with ANA serotype.
Study context
- Population
- Endothelial cells cultured with purified IgG from patients with systemic sclerosis (SSc), patients with systemic lupus erythematosus (SLE) or healthy controls
- Comparison
- Endothelial cells (EC) cultured with purified IgG from SSc vs SLE vs healthy controls (HC)
- Outcome
- EC proteome and transcriptome (PCA)
Methodological detailsderivation and external validation cohorts, LC-MS/MS proteomics, RNA sequencing
- Derivation and external validation cohorts
- LC-MS/MS proteomics
- RNA sequencing
Main finding
As reported in the abstract
- Derivation cohort proteomics principal component analysis (PCA): three distinct groups — mostly ATA+ patients; mostly anti-centromere+ patients; anti-RNA polymerase-III+ patients, SLE and HC
- Transcriptomics PCA: one group of ATA+ patients only vs a second group mixing ATA+ patients with other individuals
- Validation cohort confirmed two groups of distinct EC proteome profiles and clinical severity in ATA+ patients
- No association between functional antibodies (FA) presence and proteomic profiles in both SSc cohorts
Original publication
Immunoglobulins G from Patients with Systemic Sclerosis Modify the Molecular Signatures of Endothelial Cells. RMD Open. Published 23 March 2025. doi:10.
Composed by AI from the published abstract and checked against it. How Briefs are made →