Lupus & antiphospholipid syndrome
Ferroptosis in peripheral blood mononuclear cells of systemic lupus erythematosus
Authors' conclusion
Ferroptosis occurred more frequently in PBMCs of SLE patients than healthy controls, including CD14+ monocytes, CD19+B cells, CD56+ NK cells, and so on, with negative association with SLE disease activity
Study context
- Population
- Untreated patients with systemic lupus erythematosus (SLE) and healthy controls; Peripheral blood mononuclear cells (PBMCs)
- Participants
- 30 vs 10
- Comparison
- SLE vs healthy controls
- SLEn = 30
- Healthy controlsn = 10reference
- Outcome
- Evidence of ferroptosis in PBMCs (intracellular Fe2+, ROS, LPO; GPX4 expression; mitochondrial morphology)
Main finding
As reported in the abstract
Ferroptosis markers in PBMCs
- Fe2+, reactive oxygen species (ROS) and lipid peroxidation (LPO) significantly higher in SLE than healthy controls in CD14+ monocytes, CD19+ B cells and CD56+ NK cells
- More prominent differences in SLE with renal involvement, liver injury and higher disease activity score
- No significant difference in GPX4 mRNA expression between SLE and healthy controls
- GPX4 protein expression significantly lower in SLE than healthy controls, negatively correlated with SLE disease activity index
- Transmission electron microscopy: typical ferroptosis features (decreased mitochondrial volume, increased mitochondrial membrane density, disappearance of mitochondrial cristae)
- SLE vs Healthy controls: P < 0.05
Original publication
Ferroptosis in peripheral blood mononuclear cells of systemic lupus erythematosus. Clin Exp Rheumatol. Published 26 April 2023. doi:10.
Composed by AI from the published abstract and checked against it. How Briefs are made →