Elevated Fab glycosylation of anti-hinge antibodies
Authors' conclusion
AHA responses are characterized by elevated levels of Fab glycosylation and highly specific neoepitope recognition, not just in RA patients but also in HCs.
Study context
- Population
- Patients with early active rheumatoid arthritis (RA) and healthy controls
- Participants
- 69 vs 97
- Exposure
- Early active RA vs healthy controls
- RAn = 69
- Healthy controls (HCs)n = 97reference
- Outcome
- AHA serum levels, specificity and Fab glycosylation for IgG1/4-hinge cleaved by matrix metalloproteinase-3, cathepsin G, pepsin or IdeS
Methodological detailsELISA and lectin affinity chromatography
- ELISA and lectin affinity chromatography
Main finding
As reported in the abstract
- AHA reactivity detected for all hinge neoepitopes in both RA and HCs
- Reactivity against CatG-IgG1-F(ab´)2s and pepsin-IgG4-F(ab´)2s more prevalent in RA
- All AHA responses showed increased Fab glycosylation levels in both RA and HCs
Observational data: associations, not effects.
Original publication
Elevated Fab glycosylation of anti-hinge antibodies. Scand J Rheumatol. Published 2 November 2021. doi:10.
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