MicroRNA and interleukin 6 interplay in the adipose tissue of rheumatoid arthritis patients
Authors' conclusion
IL-6 mRNA production in AT has a negative effect on the miRs expression profile and it increases miR-production capacity.
Study context
- Population
- Subcutaneous adipose tissue (AT) of patients with rheumatoid arthritis (RA)35 AT samples on microarray
- Participants
- 75 participants
- Comparison
- AT with high vs low IL-6 mRNA; in vitro IL-6 vs IL-6 + tocilizumab
- Outcome
- Relationship between IL-6 production and miRs in AT
Methodological detailsqPCR, 3D microarray, in silico target prediction (DIANA), in vitro stimulation, Seven AT samples stimulated in vitro with IL-6 or IL-6 + tocilizumab
- qPCR
- 3D microarray
- In silico target prediction (DIANA)
- In vitro stimulation
- Seven AT samples stimulated in vitro with IL-6 or IL-6 + tocilizumab
Main finding
As reported in the abstract
- Differentially expressed (DE) microRNAs (miRs) between AT with high and low IL-6 mRNA
- 30
- DE miRs inversely related with IL-6 levels
- 26
- High IL-6 associated with higher Dicer expression
- p=0.04
- High IL-6 associated with higher Drosha expression
- p=0.04
- Tocilizumab decreased total miR-processing enzymes (in vitro)
- p=0.003
Further findings
Predicted pathways of DE miRs (in silico)
- Oestrogen signalling
- P = 0.001
- FoxO signalling
- P = 0.006
- Insulin signalling
- P = 0.03
Original publication
MicroRNA and interleukin 6 interplay in the adipose tissue of rheumatoid arthritis patients. Clin Exp Rheumatol. Published 15 October 2021. doi:10.
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