Citrullinated and carbamylated proteins in extracellular microvesicles from plasma of patients with rheumatoid arthritis
Authors' conclusion
The results of this study suggest an involvement of EMVs in the pathogenesis of RA by inducing autoimmunity.
Study context
- Population
- RA patients naïve for biological therapy and age- and sex-matched healthy donors
- Participants
- 24 vs 20
- Comparison
- RA vs healthy donors (HD)
- RAn = 24
- HDn = 20reference
- Outcome
- Expression of citrullinated and carbamylated proteins in plasma EMVs
Methodological detailscase-control, age- and sex-matched
- Case-control
- Age- and sex-matched
Main finding
As reported in the abstract
- Significant increase of extracellular microvesicles (EMVs) in RA vs HD (NanoSight)
- Significantly higher expression of citrullinated antigens on EMV surface in RA vs donors (flow cytometry)
- No substantial difference in expression of carbamylated antigens
- Western blot: vimentin, alpha-enolase 1 and collagen type II the main citrullinated and carbamylated proteins carried by EMVs
- Relevant correlation between citrullinated antigen expression and disease activity
Original publication
Citrullinated and carbamylated proteins in extracellular microvesicles from plasma of patients with rheumatoid arthritis. Rheumatology (Oxford). Published 18 October 2022. doi:10.
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