Cell and transcriptomic diversity of infrapatellar fat pad during knee osteoarthritis
Authors' conclusion
Overall, by employing a multiomic approach, this study provides the first comprehensive map of the cellular and transcriptomic diversity of human IFP and identifies IFP fibroblasts as key cells contributing to transcriptomic and metabolic differences related to KOA disease, sex or obesity.
Study context
- Population
- Human infrapatellar fat pads (infrapatellar fat pad (IFPs)) from knee osteoarthritis (KOA) and healthy control donors
- Participants
- 21 IFPs
- Comparison
- KOA vs healthy control donor IFPs; female vs male and obese vs normal body mass index (BMI) KOA-IFPs
- Healthy controln = 6reference
- KOAn = 15
Methodological detailssingle-nucleus RNA sequencing, spatial transcriptomics, metabolomics, multiomic
- Single-nucleus RNA sequencing
- Spatial transcriptomics
- Metabolomics
- Multiomic
Main finding
As reported in the abstract
- Multiple subclusters of fibroblasts, macrophages, adipocytes and endothelial cells with unique transcriptomic profiles identified
- Spatial transcriptomics resolved distributions of cell types and their transcriptomic profiles; putative cell-cell communication networks computationally identified
- Transcriptomic differences in fibroblasts: KOA vs healthy control IFPs, female vs male KOA-IFPs, obese vs normal BMI KOA-IFPs
- Differences in metabolite levels in supernatants of naïve, profibrotic and proinflammatory stimuli-treated fibroblasts from obese vs normal BMI KOA-IFPs
Original publication
Cell and transcriptomic diversity of infrapatellar fat pad during knee osteoarthritis. Ann Rheum Dis. Published 2 January 2025. doi:10.
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