CD4+CD8αlow T Cell Clonal Expansion Dependent on Costimulation in Patients With Rheumatoid Arthritis
Authors' conclusion
In patients with RA, clonal expansion of CD4+ T cell culminates in the emergence of peripheral CD4+CD8αlow T cells, which are associated with disease activity and diminished upon abatacept treatment and could contribute to disease pathogenesis.
Study context
- Population
- Patients with rheumatoid arthritis (RA), patients with PsA and healthy donors; Single-cell RNA (scRNA)/T cell receptor (TCR) sequencing in RA and healthy controlsscRNA/TCR sequencing in RA 3 · healthy controls 2
- Participants
- 53 / 52 / 50
- Comparison
- RA vs PsA vs healthy donors; RA before vs after abatacept for 12 weeks
- RAn = 53
- PsAn = 52
- Healthy donorsn = 50reference
- Outcome
- Frequency and clonality of peripheral CD4+CD8+ T cells and their subpopulations
Methodological detailssingle-cell RNA and TCR sequencing, flow cytometry, prospective abatacept cohort
- Single-cell RNA and TCR sequencing
- Flow cytometry
- Prospective abatacept cohort
Main finding
As reported in the abstract
Peripheral CD4+CD8αlow T cells
- Increase of CD4+ T cells expressing CD8α in RA vs both PsA and healthy controls
- CD4+CD8αlow T cells are part of large T cell clones, clustering separately from CD4+CD8- T cell clones in scRNA sequencing (scRNA-seq) gene expression analysis
Further findings
Frequency of peripheral CD4+CD8αlow T cells with abatacept · 12 weeks
- Abatacept significantly reduced frequency of peripheral CD4+CD8αlow T cells, linked to reduction in disease activity
Original publication
CD4+CD8αlow T Cell Clonal Expansion Dependent on Costimulation in Patients With Rheumatoid Arthritis. Arthritis Rheumatol. Published 4 September 2024. doi:10.
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