Autoantigen Peptide‐8‐Arm Polyethylene Glycol‐Dyes Specifically Recognize Antigen‐Specific B Cells and Improve the Diagnosis of Rheumatoid Arthritis
Authors' conclusion
These findings indicated APP-dyes as an effective and clinically applicable tool for investigating the mechanisms underlying antigen-specific B cells and distinguishing RA.
Study context
- Population
- Patients with rheumatoid arthritis (RA), experimental arthritis mice and spontaneous arthritis mice
- Comparison
- Mutated citrullinated vimentin-specific B cells vs Citrullinated Type II collagen-specific B cells
- Outcome
- Diagnostic performance for RA
Methodological detailsflow cytometry, in vitro culture and adoptive transfer assays
- Flow cytometry
- In vitro culture and adoptive transfer assays
Performance
As reported in the abstract
| Test or model | Sensitivity | Specificity |
|---|---|---|
| MCV-specific B cells | 73.33% | 92.5% |
| Cit-CII-specific B cells | 60.00% | 91.25% |
Sensitivity
MCV-specific B cells73.33%
Cit-CII-specific B cells60.00%
Specificity
MCV-specific B cells92.5%
Cit-CII-specific B cells91.25%
Further findings
Diagnostic performance in anti-CCP-negative and early RA
- Substantial diagnostic performance in anti-CCP-negative and early patients with RA
Antigen-specific B cells combined with corresponding autoantibodies
- Combination further increased diagnostic accuracy
Original publication
Autoantigen Peptide‐8‐Arm Polyethylene Glycol‐Dyes Specifically Recognize Antigen‐Specific B Cells and Improve the Diagnosis of Rheumatoid Arthritis. Arthritis Rheumatol. Published 28 November 2025. doi:10.
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