Assessing the sensitivity and specificity of myositis-specific and associated autoantibodies: a sub-study from the MyoCite cohort
Authors' conclusion
While LIA reliably detected anti-Jo1, anti-PL7, anti-PL12, anti-MDA5 and anti-NXP-2, it also displayed false positives and negatives. Its effectiveness in detecting other autoantibodies, such as anti-TIF1γ, was poor.
Study context
- Population
- Adult and juvenile idiopathic inflammatory myopathy (IIM) patients in the MyoCite cohort, Lucknow, India (2017–2020)125 adult-onset, 22 juvenile-onset
- Participants
- 147 IIM patients' serum samples
- Comparison
- Line immunoassay vs protein radio-immunoprecipitation
- Outcome
- LIA performance against immunoprecipitation: sensitivity, specificity and inter-rater agreement
Methodological detailssub-study of the MyoCite cohort, retro-prospective, assessors blind to line immunoassay (LIA) results
- Sub-study of the MyoCite cohort
- Retro-prospective
- Assessors blind to LIA results
Performance
As reported in the abstract
Agreement of LIA with immunoprecipitation
| Test or model | Cohen's κ anti-Jo1 | Cohen's κ anti-PL7 | Cohen's κ anti-PL12 | Cohen's κ anti-TIF1γ | κ range anti-TIF1γ, anti-Ku, anti-PmScl, anti-Mi2, anti-SAE |
|---|---|---|---|---|---|
| LIA | 0.79 | 0.83 | 1 | 0.21 | 0.21 and 0.60 |
LIA
Cohen's κ anti-Jo10.79
Cohen's κ anti-PL70.83
Cohen's κ anti-PL121
Cohen's κ anti-TIF1γ0.21
κ range anti-TIF1γ, anti-Ku, anti-PmScl, anti-Mi2, anti-SAE0.21 and 0.60
- Samples non-congruent on LIA and immunoprecipitation
- 59 (40.1%)
Further findings
| Outcome | Values |
|---|---|
| Myositis autoantibodies identified by immunoprecipitation | Immunoprecipitation 56.5% · anti-Jo1 (most common) n = 16; 10.9% · anti-MDA5 n = 14, 9.5% |
Parts of this Brief are withheld: the automatic checks could not confirm them against the abstract.
Original publication
Assessing the sensitivity and specificity of myositis-specific and associated autoantibodies: a sub-study from the MyoCite cohort. Rheumatology (Oxford). Published 13 March 2024. doi:10.
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