Anti‐Inflammatory Effect of High‐Density Lipoprotein Blunted by Delivery of Altered MicroRNA Cargo in Patients With Rheumatoid Arthritis
Authors' conclusion
HDL-miR-1246 was increased in RA versus control participants and delivery of miR-1246 to macrophages increased IL-6 expression by targeting DUSP3. The altered HDL-miRNA cargo in RA blunted HDL's anti-inflammatory effect.
Study context
- Population
- Rheumatoid arthritis (RA) and control participants; monocyte-derived macrophages treated with miR-1246-loaded high-density lipoprotein (HDL) and unmodified HDL
- Participants
- 30 vs 30
- Comparison
- HDL-miRNA cargo in RA vs control participants
- RAn = 30
- Controln = 30reference
- Outcome
- Whether HDL-miRNA cargo is altered and affects inflammation in RA
Methodological detailsnext generation sequencing, quantitative polymerase chain reaction, miRNA mimics, antagomiRs, small interfering RNA knockdown and luciferase assays
- Next generation sequencing
- Quantitative polymerase chain reaction
- miRNA mimics, antagomiRs, small interfering RNA knockdown and luciferase assays
Main finding
As reported in the abstract
- Most abundant miRNA on HDL
- miR-1246
- miR-1246 enrichment on HDL, RA vs control (significant)
- two-fold
- IL6 expression increase with HDL-mediated miR-1246 delivery to macrophages (significant)
- 43-fold
- DUSP3 decrease with miR-1246 delivery (significant); DUSP3 siRNA knockdown increased macrophage IL6 expression
- 1.5-fold
- miR-1246 delivered by unmodified HDL, RA vs control
- 1.6-fold more
- Activated macrophage IL6 expression after RA vs control HDL treatment (HDL's attenuating effect significantly blunted in RA)
- 3.4-fold higher
Original publication
Anti‐Inflammatory Effect of High‐Density Lipoprotein Blunted by Delivery of Altered MicroRNA Cargo in Patients With Rheumatoid Arthritis. Arthritis Rheumatol. Published 24 January 2024. doi:10.
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