Altered gut microbiota and host pathways in obesity-related knee osteoarthritis
Authors' conclusion
OB+OA+ patients exhibited significant gut microbial dysbiosis associated with systemic metabolic and proteomic alterations relevant to OA pathophysiology.
Study context
- Population
- Knee osteoarthritis (OA) patients and OA-free controls, classified by obesity and OA status91 knee OA, 12 controls
- Participants
- 66 / 25 / 5 / 7
- Exposure
- Obesity (OB)+OA+ vs OB-OA+
- OB+OA+n = 66
- OB-OA+n = 25reference
- OB+OA-n = 5
- OB-OA-n = 7
- Outcome
- Gut microbiota alterations in OB+OA+
Methodological detailsmulti-omics integration (16S rRNA microbiome, plasma metabolomics, proteomics, dietary data), MaAsLin2 modelling, Plasma metabolomics (n=630 metabolites) and proteomics (n=5,416 proteins) integrated with microbial signatures
- Multi-omics integration (16S rRNA microbiome, plasma metabolomics, proteomics, dietary data)
- MaAsLin2 modelling
- Plasma metabolomics (n=630 metabolites) and proteomics (n=5,416 proteins) integrated with microbial signatures
Main finding
As reported in the abstract
Microbial taxa significantly associated with OB+OA+ (all p<7.65×10-5 after correcting tests for 654 ASVs)
17
Taxa remaining significant after adjustment for age, sex, antibiotic use and dietary intake
16
Metabolites correlated with these taxa (p<0.05); enrichment in fatty acid biosynthesis, linoleic/arachidonic acid metabolism, propanoate metabolism
376
Proteins associated with these taxa (p<0.001); enrichment in PI3K-Akt signalling, ECM-receptor interaction, lipid/atherosclerosis
146
Observational data: associations, not effects.
Original publication
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