Agreement between local and central anti-synthetase antibodies detection: results from the Classification Criteria of Anti-Synthetase Syndrome project biobank
Authors' conclusion
Our analysis reinforces the reliability of real-world anti-Jo1 detection methods. In contrast, challenges persist for anti-non-Jo1 identification, particularly anti-PL7 and rarer antibodies such as anti-OJ/KS.
Study context
- Population
- Multi-national patients with anti-synthetase syndrome (ASSD) and controls from centres participating in the CLASS project
- Participants
- 787 serum samples
- Comparison
- Local anti-ARS immunoassays vs central definition of anti-ARS positivity
- Outcome
- Sensitivity, specificity, positive likelihood ratio, PPV and NPV of local anti-ARS testing, and agreement with central results (weighted κ)
Methodological detailsinternational multicentre, ad-interim study within the CLASS project, central reference: RNA-IP, reconfirmed by ELISA, LIA and protein-IP
- International multicentre
- Ad-interim study within the CLASS project
- Central reference: RNA-IP, reconfirmed by ELISA, LIA and protein-IP
Performance
As reported in the abstract
Agreement of local vs central anti-ARS detection
- Anti-Jo1: local detection reliable, with high sensitivity and specificity and a very good level of agreement with central definition
- Anti-non-Jo1: agreement between local immunoassay and central determination varied, especially for local LIA, ELISA and "other" methods
Original publication
Agreement between local and central anti-synthetase antibodies detection: results from the Classification Criteria of Anti-Synthetase Syndrome project biobank. Clin Exp Rheumatol. Published 14 March 2024. doi:10.
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