Systemic sclerosis & Raynaud's
Adeno‐Associated Virus Type 5 Infection via PDGFRα Is Associated With Interstitial Lung Disease in Systemic Sclerosis and Generates Composite Peptides and Epitopes Recognized by the Agonistic Immunoglobulins Present in Patients With Systemic Sclerosis
Authors' conclusion
These data link AVV5 with the immune reactivity to endogenous antigens in SSc and provide a novel element in the pathogenesis of SSc.
Study context
- Population
- Patients with systemic sclerosis (SSc) with ILD and controls; SSc lung tissue, bronchoalveolar lavage (BAL) fluid and sera
- Participants
- 66 vs 66
- Comparison
- AAV5 detection in BAL fluid of SSc patients with ILD vs controls
- SSc WITH ILDn = 66
- CONTROLSn = 66reference
- Outcome
- AAV5 detection in BAL fluid
Methodological detailsin silico molecular docking, surface plasmon resonance, in situ hybridisation, immunohistochemistry, confocal microscopy
- In silico molecular docking
- Surface plasmon resonance
- In situ hybridisation, immunohistochemistry, confocal microscopy
Main finding
As reported in the abstract
AAV5 detected in BAL fluid
P < 0.001
Further findings
AAV5 localisation in SSc lungs
- AAV5 localised in type II pneumocytes and in interstitial cells
AAV5 capsid–PDGFRα molecular complex
- Molecular complex of spatially contiguous epitopes of the AAV5 capsid and of PDGFRα identified and characterised
Binding of composite epitopes to agonistic anti-PDGFRα antibodies
- Spatially contiguous epitopes derived from PDGFRα and AAV5 interacted with SSc agonistic antibodies to PDGFRα
Binding of peptides to total IgG
- Peptides bound total IgG isolated from patients with SSc, not from healthy controls
Original publication
Adeno‐Associated Virus Type 5 Infection via PDGFRα Is Associated With Interstitial Lung Disease in Systemic Sclerosis and Generates Composite Peptides and Epitopes Recognized by the Agonistic Immunoglobulins Present in Patients With Systemic Sclerosis. Arthritis Rheumatol. Published 15 February 2024. doi:10.
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