Unveiling inflammatory and prehypertrophic cell populations as key contributors to knee cartilage degeneration in osteoarthritis using multi-omics data integration
Authors' conclusion
Our results highlight InfC, preHTC, preFC and HTC as potential cell populations to target for therapy.
Study context
- Population
- Human knee articular cartilage tissue from osteoarthritis (OA) and non-OA donors19 samples
- Participants
- 8 vs 3
- Comparison
- OA donors vs non-OA control donors
- OAn = 8
- Non-OA controln = 3reference
- Outcome
- Cellular heterogeneity of knee articular cartilage
Methodological detailssingle-cell RNA sequencing, spatially resolved transcriptomics, integrative analyses of bulk RNA sequencing data and genome-wide association studies, Novel chondrocyte population and marker genes validated by immunohistochemistry staining and quantitative real-time PCR
- Single-cell RNA sequencing
- Spatially resolved transcriptomics
- Integrative analyses of bulk RNA sequencing data and genome-wide association studies
- Novel chondrocyte population and marker genes validated by immunohistochemistry staining and quantitative real-time PCR
Main finding
As reported in the abstract
Cell population-specific marker genes identified
33
Chondrocyte populations defined (9 known, 2 new: preInfC and InfC)
11
Original publication
Unveiling inflammatory and prehypertrophic cell populations as key contributors to knee cartilage degeneration in osteoarthritis using multi-omics data integration. Ann Rheum Dis. Published 12 June 2024. doi:10.
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