TWEAK and Fn14 are overexpressed in immune-mediated necrotizing myopathy: implications for muscle damage and repair
An erratum to this article was published (Rheumatology (Oxford), 2 Nov 2023 ↗)
Authors' conclusion
TWEAK/Fn14 signalling was activated in IMNM, most likely aggravating muscle damage via amplifying inflammatory response and macrophages chemotaxis. Fn14 seems to be a biomarker for assessing disease severity in IMNM.
Study context
- Population
- Patients with immune-mediated necrotizing myopathy (IMNM) and controls; muscle biopsies, serum and cultured human muscle cells
- Participants
- 37 vs 11
- Comparison
- IMNM muscle biopsies vs controls
- IMNMn = 37
- Controlsn = 11reference
- Outcome
- Role of TWEAK/Fn14 in IMNM
Methodological detailsin vitro TWEAK treatment of human muscle cells, immunostaining and quantitative PCR, serum ELISA
- In vitro TWEAK treatment of human muscle cells
- Immunostaining and quantitative PCR
- Serum ELISA
Main finding
As reported in the abstract
- TWEAK and Fn14 overexpressed in IMNM muscle biopsies
- % Fn14-positive myofibers correlated with disease severity, myonecrosis, regeneration and inflammation infiltrates
- Fn14-positive myofibers tended to be surrounded or invaded by CD68+ macrophages
- TWEAK treatment harmful to cultured muscle cells, inducing multiple chemokines and pro-inflammatory cytokines
- Serum Fn14 increased in IMNM and correlated with muscle weakness
Original publication
TWEAK and Fn14 are overexpressed in immune-mediated necrotizing myopathy: implications for muscle damage and repair. Rheumatology (Oxford). Published 14 March 2023. doi:10.
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