Synovial fluid potentiates local fibroblasts to drive monocyte activation in juvenile idiopathic arthritis
Authors' conclusion
Our data show a contact dependent role for S-Fib in driving inflammation in oJIA by inducing pro-inflammatory monocytes, processes potentiated by inflamed SF.
Study context
- Population
- Synovial fibroblasts from patients with oligoarticular JIA and rheumatoid arthritis (RA), and healthy adult synovial fibroblasts, co-cultured with monocytes from healthy donors
- Comparison
- Synovial fibroblasts (S-Fib) from oligoarticular juvenile idiopathic arthritis (oJIA), RA or healthy donors co-cultured with healthy monocytes; S-Fib primed with pooled oJIA synovial fluid (SF) vs S-Fib alone
- oJIA S-Fibn = 13
- RA S-Fibn = 4
Methodological detailsin vitro co-culture
- In vitro co-culture
Main finding
As reported in the abstract
- Co-culture with S-Fib induced inflammatory activation of healthy monocytes (increased CD86 and HLA-DR expression, cytokine production, subsequent T-cell activation)
- Only minor differences in monocyte activation by S-Fib source (oJIA, RA or healthy)
- Priming of S-Fib with pooled SF vs S-Fib alone induced an inflammatory phenotype, IL-6 production and enhanced monocyte chemotaxis
- Primed S-Fib induced a more pronounced inflammatory phenotype in healthy monocytes vs S-Fib alone
- Direct cell-cell contact between S-Fib and monocytes required for full induction of monocyte activation
Original publication
Synovial fluid potentiates local fibroblasts to drive monocyte activation in juvenile idiopathic arthritis. Arthritis Res Ther. Published 28 February 2026. doi:10.
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