Lupus & antiphospholipid syndrome
Single-cell transcriptome analyses reveal disturbed decidual homoeostasis in obstetric antiphospholipid syndrome
Authors' conclusion
This study provided a comprehensive decidual cell landscape and identified aberrant decidual microenvironment in OAPS
Study context
- Population
- First-trimester decidua from patients with obstetric APS and healthy controlsplus larger validation cohort
- Participants
- 5 vs 5
- Comparison
- Obstetric APS vs healthy controls
- Obstetric antiphospholipid syndrome (OAPS)n = 5
- Healthy controls (HCs)n = 5reference
- Outcome
- Cellular compositions and molecular features of decidual cells (single-cell RNA sequencing (scRNA-seq))
- Follow-up
- First trimester
Methodological detailssingle-cell RNA sequencing, validation with flow cytometry, immunohistochemical staining and immunofluorescence in a larger cohort
- Single-cell RNA sequencing
- Validation with flow cytometry, immunohistochemical staining and immunofluorescence in a larger cohort
Main finding
As reported in the abstract
Decidual cell composition and molecular features
- Higher ratio of macrophages but lower ratio of decidual natural killer (dNK) cells in OAPS vs HCs
- Vascular endothelial cells shrank in OAPS decidua, with upregulated chemokine expression and conspicuous responses to IFN-γ and TNF-α
- OAPS macrophages: stronger phagocytosis, complement activation signals, more reliance on glycolysis
- OAPS dNK cells more activated, with enhanced cytotoxicity and IFN-γ production; downregulated granules possibly associated with suppressed glycolysis
- Stromal cells in prosenescent state with weakened immune surveillance for senescent cells in OAPS
- Altered cellular interactions among immune cells and between immune and non-immune cells, especially via chemokines, IFN-γ and TNF-α
Original publication
Single-cell transcriptome analyses reveal disturbed decidual homoeostasis in obstetric antiphospholipid syndrome. Ann Rheum Dis. Published 11 April 2024. doi:10.
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