Single-cell landscape of peripheral immune response in patients with anti-melanoma differentiation–associated gene 5 dermatomyositis
Authors' conclusion
Our study has illustrated the peripheral immune cell atlas of a number of DM subtypes, has provided compelling evidence for a viral infection-derived origin for anti-MDA5 DM, and has indicated potential targets for innovative therapeutic interventions.
Study context
- Population
- Patients with DM, including anti-MDA5-positive DM, and healthy controls; Peripheral blood mononuclear cells (PBMCs)plus 4 external healthy donors
- Participants
- 8 vs 2
- Comparison
- DM patients vs healthy donors
- DMn = 8
- Healthy donorsn = 2reference
- Outcome
- Peripheral immune landscape in anti-MDA5 DM (scRNA-seq)
Methodological detailssingle-cell RNA sequencing, 10X Genomics platform
- Single-cell RNA sequencing
- 10X Genomics platform
Main finding
As reported in the abstract
- Aberrantly increased proportions of CD14+ monocytes and plasma cells in anti-MDA5 DM PBMC samples
- Overactivated type I IFN response and antiviral immunity in innate and adaptive immune cells from anti-MDA5 DM patients, positively correlated with disease severity
- Unique subset of CD14+ monocytes highly expressing IFI27 (viral infection biomarker) and IFIH1 (encodes MDA5) specifically identified in anti-MDA5 DM samples
Original publication
Single-cell landscape of peripheral immune response in patients with anti-melanoma differentiation–associated gene 5 dermatomyositis. Rheumatology (Oxford). Published 6 November 2023. doi:10.
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