Rheumatoid arthritis synovial fluid shows enrichment of T-cells producing GMCSF which are polyfunctional for TNFα and IFNγ
Authors' conclusion
We report a novel finding of enrichment of CD8+GMCSF+ in addition to CD4+GMCSF+ T-cells in RA-SF.
Study context
- Population
- Rheumatoid arthritis (RA) patients (blood, some with paired synovial fluid (SF)), healthy controls (blood) and SpA patients (SF)
- Comparison
- RA synovial fluid vs RA blood; RA blood vs healthy controls (HC) blood
- Outcome
- Frequencies of CD8+GMCSF+ T cells in RA blood and SF, with polyfunctionality, memory phenotype and cytotoxic ability
Methodological detailsex-vivo stimulation (PMA/Ionomycin), flow cytometry, longitudinal follow-up before and after methotrexate (MTX)
- Ex-vivo stimulation (PMA/Ionomycin)
- Flow cytometry
- Longitudinal follow-up before and after MTX
Main finding
As reported in the abstract
- GMCSF+CD8+ T-cells, RA vs HC blood (no significant difference)
- 3.7, 4.1%, p=0.540
- GMCSF+CD4+ T-cells, RA vs HC blood (no significant difference)
- 4.5, 5.2%, p=0.450
- CD8+GMCSF+ T-cells enriched in SF vs blood in RA
- 5.8, 3.9%, p=0.0045
- CD4+GMCSF+ T-cells enriched in SF vs blood in RA
- 8.5, 4.5%, p=0.0008
- Triple-positive TNFα+IFNγ+GMCSF+CD8+ T-cells higher in SF vs blood in RA
- 81, 36%, p=0.049
- Triple-positive TNFα+IFNγ+GMCSF+CD4+ T-cells higher in SF vs blood in RA
- 48, 32%, p=0.010
Further findings
Blood CD4+GMCSF+ T-cells after MTX (longitudinal follow-up) · 16-24 weeks after MTX
- Significant decline post-MTX
- 4.6, 2.9%, p=0.0014
Original publication
Rheumatoid arthritis synovial fluid shows enrichment of T-cells producing GMCSF which are polyfunctional for TNFα and IFNγ. Clin Exp Rheumatol. Published 2 September 2023. doi:10.
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