Myositis-specific autoantibodies recognising Mi2 also target the autoimmune regulator (AIRE) protein at a shared PHD zinc finger
Authors' conclusion
Autoantibodies targeting Mi2 recognise AIRE at a shared PHD1 epitope - a conserved motif found in numerous transcriptional regulators.
Study context
- Population
- Patients with anti-Mi2 autoantibody-positive myositis; healthy controls, patients with myositis and those with other autoimmune diseases
Main finding
As reported in the abstract
- Phage immunoprecipitation sequencing (PhIP-Seq) identified autoantibodies recognising AIRE in sera from anti-Mi2 autoantibody-positive patients
- Both anti-AIRE and anti-Mi2 autoantibodies predominantly recognised a homologous region containing PHD1, critical for AIRE and Mi2/NuRD function
- ELISA and luciferase immunoprecipitation system (LIPS): anti-Mi2-positive patients were positive for anti-AIRE autoantibodies; AIRE reactivity largely absent in healthy comparators, anti-Mi2-negative myositis and other autoimmune diseases
- Affinity-purified anti-Mi2 autoantibodies recognised both Mi2 and AIRE by ELISA; anti-Mi2-depleted fractions recognised neither protein
Original publication
Myositis-specific autoantibodies recognising Mi2 also target the AIRE protein at a shared PHD zinc finger. Ann Rheum Dis. Published 15 September 2025. doi:10.
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