Systemic sclerosis & Raynaud's
Imaging mass cytometry-based characterisation of fibroblast subsets and their cellular niches in systemic sclerosis
Authors' conclusion
Using IMC, we demonstrated profound changes in composition and localisation of the majority of fibroblast subpopulations in SSc skin.
Study context
- Population
- Skin sections of patients with systemic sclerosis (SSc) and controls49,969 cells incl
- Comparison
- SSc skin vs control skin
- Outcome
- Fibroblast subpopulations, spatial distribution and cellular niches by imaging mass cytometry (IMC)
Methodological detailsimaging mass cytometry, single-cell, spatially resolved
- Imaging mass cytometry
- Single-cell
- Spatially resolved
Main finding
As reported in the abstract
Fibroblast subpopulations in SSc vs control skin
Fibroblast subpopulations identified in SSc and control skin
13
Subpopulations with increased proportion in SSc (myofibroblasts, FAPhigh, S1PR+, Thy1+;ADAM12high;PU.1high, ADAM12+;GLI1+)
5
Subpopulations with decreased proportion in SSc (TFAMhigh, PI16+;FAP+, Thy1+;ADAM12low)
3
Further findings
Association with extent of skin fibrosis in SSc
- S1PR+ fibroblast proportion positively correlated with more extensive skin fibrosis
- High numbers of PI16+;FAP- fibroblasts associated with milder skin fibrosis
- Frequency of aberrant S1PR+ and ADAM12+;GLI1+ fibroblast interaction positively associated with extent of skin fibrosis
Original publication
Imaging mass cytometry-based characterisation of fibroblast subsets and their cellular niches in systemic sclerosis. Ann Rheum Dis. Published 23 October 2024. doi:10.
Composed by AI from the published abstract and checked against it. How Briefs are made →