Heightened Proinflammatory Glycosylation of Borrelia burgdorferi IgG Antibodies in Synovial Fluid in Patients With Antibiotic‐Refractory Lyme Arthritis
Authors' conclusion
Patients with oral antibiotic-responsive LA had Bb IgG1 antibodies with more balanced proinflammatory/antiinflammatory glycan profiles, whereas patients with antibiotic-refractory LA had Bb IgG1 antibodies with maximal, minimally opposed, proinflammatory glycan profiles.
Study context
- Population
- Patients with Lyme arthritis; serum and synovial fluid samples
- Participants
- 21 patients
- Comparison
- Antibiotic-responsive vs antibiotic-refractory Lyme arthritis
- Outcome
- Glycan profiles of total IgG1 and Bb-specific IgG1 antibodies and their correlation with treatment outcome
Methodological detailsGlycoblotting with hydrazide glycan enrichment and MALDI-TOF mass spectrometry after affinity-column processing
- Glycoblotting with hydrazide glycan enrichment and MALDI-TOF mass spectrometry after affinity-column processing
Main finding
As reported in the abstract
- Antibiotic-responsive Lyme arthritis (LA) vs refractory LA: total and Borrelia burgdorferi (Bb)-specific IgG1 glycans with less intense inflammatory profiles (lower % GlcNAc and bisecting GlcNAc, higher % galactose and fucose)
- Refractory LA before IV antibiotics: maximal, minimally opposed, proinflammatory glycan profiles (high % GlcNAc and bisecting GlcNAc, intermediate % galactose and fucose, low % sialic acid)
- Refractory LA seen after IV antibiotics: Bb IgG1 with strongly inflammatory glycan profiles, but inflammatory potential appeared to be waning
Original publication
Heightened Proinflammatory Glycosylation of Borrelia burgdorferi IgG Antibodies in Synovial Fluid in Patients With Antibiotic‐Refractory Lyme Arthritis. Arthritis Rheumatol. Published 11 May 2023. doi:10.
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